Institute of medicine of medicine of traditional Chinese medical science of Henan of Nanjing medicine institute
One, small rat heats up board law analgesia to experiment
Adjust constant temperature implement water is warm, make its board temperature maintains in 55 ± 0.5 ℃ , room temperature control is in 18 ℃ of 15 ～ , weight the female small rat of 20 ± 2g is put into metallic canister inside, determine each fundamental threshold of pain of small rat before medicine, choose threshold of pain to all be worth the small rat in 5S-30S 60, random cent is 3 groups, every groups 20, the " rich that skin of experimental group abdomen besmears put on the skin uses physiological saline dilute gives birth to cancer to appear leather treatment to stick " liquid extract rather, 1g/ml, 1ml/10g; positive contrasts group to Du Lengding abdominal cavity injects, 0.1mg/10g; Blank contrasts group to wait for capacity physiological saline, 0.5ml/10g, put on the skin of abdominal skin besmear. At giving medical hind 15, 30, 45 Min measures threshold of pain 3 times, take its to all be worth, experimental result classics is statistical processing, detailed sees a table 1:
Unripe cancer of rich of watch 1 " appears leather treatment to stick " bandicoot report to stimulate law analgesia to experiment rather (N ＝ 20)
Group fasten fundamental threshold of pain (X ± SD) the threshold of pain after giving drug (S, x ± SD)
15min 30min 45min
Du Lengding positive contrasts group of 3.8 ± 4.89** of ± of 9.18** 7.8 of ± of 11.43** 15.6 of 1.2 23.8 ±
Group of peaceful test of rich unripe cancer 17.2 ± of 9.97** Δ of ± of 4 ± 1.6 19.74 14.5 ± of 11.01** Δ 7.18** Δ Δ
** and fundamental threshold of pain compare: P<0.01; Δ and positive contrast group quite,
Δ of P>0.05; Δ and positive contrast group quite, p<0.05
Experimental result makes clear: "Rich unripe cancer appears leather treatment to stick " and Du Lengding to all can raise bandicoot threshold of pain apparently rather, the threshold of pain when rich gives birth to 15min of cancerous peaceful group is 19.74 ± 9.97, the threshold of pain when 30min is 17.2 ± 11.01, the threshold of pain when 45min is 14.5 ± 7.18, contrast with Du Lengding group of photograph comparing, p ＞ 0.05 with P ＜ 0.05.
2, analgesia of law of bandicoot report stimulation experiments
Take weight the bandicoot of 150 ± 10g, male and female holds concurrently with. Chase first determine only the fundamental threshold of pain of each bandicoot before medicine, be in bandicoot rear glue of electric conduction of Tu Yibao layer, with pharmacodynamics physiology multi-purpose appearance undertakes successive stimulation, frequency 8Hz, wave wide 2ms, voltage from 0 begin, the record gives out the voltage when bray of high and level tone to be worth after bandicoot report is exciting, determine 3 times, take its to all be worth the threshold of pain before be medicine, choice foundation threshold of pain is worth the bandicoot in 10S less than 40, random cent is two groups, every groups 20, the " rich that besmear of skin of experimental group abdomen uses physiological saline dilute gives birth to cancer to appear leather treatment to stick " liquid extract rather, 1g/ml, 1ml/10g; positive contrasts group inject to dolantin abdominal cavity, 0.1mg/10g. After measuring medicine respectively 15, 30, the threshold of pain when 45min, with before giving drug, compare, experimental result sees a table 2.
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