Beijing of (of Chen Ming of college of medicine of foundation of university of medicine of Beijing traditional Chinese medical science 100029)
　　
Keyword: 100 blessing give birth to wither of cell of cancer of the stomach of capsule tumour cancer to die
The biology reaction conditioning agent that 100 blessing give birth to capsule department to a kind of natural medicaments is extracted and be become (Biological Response Modify -- BMS) , clinical and main effect enrages filling empty for beneficial, fu Zhengzeng is avoided, improve immune function. In recent years consider to make clear, 100 blessing unripe capsule is had fight tumor action, inside body, experiment of tumour of the curb outside body shows unripe extraction fluid has 100 blessing to combat tumor effect apparently, can lengthen small rat of tumour of carry on one's shoulder live period [1] , the T cell that clinical research shows to 100 blessing give birth to capsule to be able to affect patient of chronic diseases marked by deficiency of vital energy and lowering of body resistance inferior group [2] , can improve patient of terminal and malignant tumor live quality, lengthen its to live period [3] , to discuss 100 blessing further unripe capsule fights the action mechanism that tumor treats, we apply 100 blessing to give birth to extraction fluid to die to the wither of cell of person cancer of the stomach action undertook the experiment studies, report as follows now:
1, material and method
1.1 tests data
Experimental drug: 100 blessing give birth to extraction fluid to give birth to medical technology to develop limited company to offer by Zhengzhou rich, chroma 98.5% , physiological saline is used first before the experiment give birth to 100 blessing preparation of extraction fluid dilute, make its eventually chroma dilute becomes 10^-5 Mol/L of 10^-4 Mol/L of 5 × 10^-3 Mol/L, 5 × , 5 × 100 blessing give birth to 3 kinds of different pH indicator extraction diluent, reserve.
Experimental reagent: Calf serum: Institute of Shanghai biological product is offerred, 56 ℃ , 30min destroys vivid, - 20 ℃ freezer is saved. PRMI1640 fosters fluid: American Gibco company is produced.
Department of cell of SGC-7901 of cancer of the stomach of person of individual plant of 1.1.2 experiments cell is brought from Beijing tumour institute.
1.1.3 experiments instrument
Optical microscope, chongqing telescope factory, electron microscope () of company of JEN-100CX Japan electron, 96 aperture are fostered board () of Danish Nunc company, FACS Vantage sheds type cell appearance () of American BECTON-DICKINSONW company.
1.2 experiments method
1.2.1 cells foster: Foster fluid with what contain streptomycin of penicillin of serum of RPMI1640, 10% calves, 100U/mL, 100mg/ml, the in suspension in the constant temperature box of 37 ℃ , 5%CO2 and saturated humidity fosters buy cell of SGC-7901 of person cancer of the stomach, every 2-3 day goes down to the future generation. In go down to the future generation the cell of 24h is dangerous 3 kinds of different pH indicator are joined in fluid 100 blessing give birth to extraction diluent (5 × 10, 3 Mol/L, 5 × 10, 4 Mol/L, 5 × 10, 5 Mol/L) , part to be measured fully at increasing cell of collect of adduction of the 24h after medicine, 48h, 72h, die in order to observe cellular wither circumstance. Set contrast group.
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